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Cyclic peptides:

1. ATP/PPi exchange for non-ribosomal peptide synthetases
2. Purification and characterization of HC-toxin, chlamydocin, depudecin, etc.
        a. growth conditions
        b. HPLC
        c. TLC (epoxide detection)

Cell wall biosynthesis:

1. growth of maize
2. assay for glucan synthase I
3. assays for sucrose gradients
 

Cell wall degrading enzymes:

1. growth of fungus
2. extraction (rotary evaporation, HPLC)
3. assay
    a. PAHBAH
    b. nitrophenol derivatives

Bradford assay for proteins:

MORE TO COME LATER...


(updated April 8, 2004)